Electron microscopy of frozen biological suspensions

Détails

Ressource 1Télécharger: 1983_Lepault_Wiley.pdf (966.10 [Ko])
Etat: Serval
Version: Final published version
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Sous embargo indéterminé.
Etat: Serval
Version: Supplementary document
ID Serval
serval:BIB_4581D1BB44BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Electron microscopy of frozen biological suspensions
Périodique
Journal of Microscopy
Auteur(s)
Lepault  J., Booy  F. P., Dubochet  J.
ISSN
0022-2720 (Print)
Statut éditorial
Publié
Date de publication
01/1983
Peer-reviewed
Oui
Volume
129
Numéro
Pt 1
Pages
89-102
Langue
anglais
Notes
Journal Article --- Old month value: Jan
Résumé
The methodology for preparing specimens in the frozen, hydrated state has been assessed using crystals and T4 bacteriophages. The methods have also been demonstrated with lambda bacteriophages, purple membrane of Halobacterium halobium and fibres of DNA. For particles dispersed in an aqueous environment, it is shown that optimum structural preservation is obtained from a thin, quench-frozen film with the bulk aqueous medium in the vitreous state. Crystallization of the bulk water may result in solute segregation and expulsion of the specimen from the film. Contrast measurements can be used to follow directly the state of hydration of a specimen during transition from the fully hydrated to the freeze-dried state and permit direct measurement of the water content of the specimen. By changing the concentration and composition of the aqueous medium the contrast of particles in a vitreous film can be controlled and any state of negative, positive or zero contrast may be obtained. At 100 K, frozen-hydrated, freeze-dried or sugar embedded crystals can withstand a three- to four-fold increase in electron exposure for the same damage when compared with similar sugar-embedded or freeze-dried samples at room temperature.
Mots-clé
Catalase Cell Membrane/ultrastructure Crystallization Dna Freeze Drying Freezing Halobacterium/ultrastructure *Microscopy, Electron *Specimen Handling Staining and Labeling T-Phages/ultrastructure Water
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
08/05/2019 17:54
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