Inducible and constitutive MHC class II gene expression. Distinct tissue-specific genetic controls.

Détails

ID Serval
serval:BIB_456CE02D399B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inducible and constitutive MHC class II gene expression. Distinct tissue-specific genetic controls.
Périodique
Journal of immunology
Auteur(s)
Sartoris S., Scupoli M.T., Scarpellino L., Paiola F., Jotterand-Bellomo M., Tridente G., Accolla R.S.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
1990
Volume
145
Numéro
6
Pages
1960-7
Langue
anglais
Résumé
B cells express MHC class II Ag in a constitutive fashion, whereas macrophages can do so only after induction by a variety of exogenous stimuli. In this study we describe interspecies somatic cell hybrids between the human B cell Raji and the murine macrophage cell P388 D1. This murine cell line does not express detectable levels of class II mRNA. Phenotypic, molecular, and karyotype analysis of a series of hybrids showed that murine macrophage class II genes can be expressed in a constitutive fashion under the control of the human B cell genome. This event is the consequence of de novo accumulation of class II specific mRNA and thus probably reflects activation of transcription. In certain cases the amount of murine class II Ag expressed on the surface of the hybrid cell was significantly higher than the one observed in the parental macrophage cells after induction with IFN-gamma and was not further modified by treatment with the murine lymphokine. Reversion from a murine class II-positive to class II-negative cell surface phenotype in the hybrids correlated with reduced expression of human markers and more important with segregation of human chromosomes. Interestingly, in this case certain hybrids still expressed detectable levels of murine class II mRNA and increased levels of murine invariant chain mRNA when compared with parental P388 D1 murine macrophage cells. These results indicate that constitutive class II gene expression behaves as a dominant trait in B cell x macrophage somatic cell hybrids. Possible mechanisms responsible of the different control of class II gene expression during cell type differentiation are discussed.
Mots-clé
Animals, B-Lymphocytes, Blotting, Northern, Flow Cytometry, Gene Expression Regulation, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Humans, Hybrid Cells, Karyotyping, Macrophages, Major Histocompatibility Complex, Mice, RNA, Messenger
Pubmed
Création de la notice
22/05/2009 10:02
Dernière modification de la notice
03/03/2018 16:45
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