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Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4.
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The consensus recognition element for the mammalian transcription factor AP-1 is very similar to that of the transcriptional activator GCN4. Here, we show that the AP-1 recognition element (ARE) found in the SV40 enhancer can activate transcription from a heterologous promoter in S. cerevisiae. This activation, however, is not dependent on the presence of GCN4 as evidenced by ARE-dependent transcription in a gcn4 yeast strain. A previously unknown yeast transcription factor that is probably responsible for this activation was identified and highly purified. The yeast factor, designated yAP-1, shares remarkably similar biochemical and DNA-binding characteristics with mammalian AP-1. These data suggest that the yeast and mammalian AP-1 are evolutionarily conserved and perhaps functionally related. Also note-worthy is that GCN4 can bind to a GCN4 recognition element (GCRE) and to the ARE with approximately equal affinities; yAP-1, however, has a much lower affinity for the GCRE than the ARE, suggesting that yAP-1 can discriminate between these elements in vivo.
Antibodies, Monoclonal, Base Sequence, Cell Nucleus/metabolism, DNA-Binding Proteins/analysis, DNA-Binding Proteins/genetics, Enhancer Elements, Genetic, Evolution, Fungal Proteins/analysis, Fungal Proteins/genetics, Genes, Fungal, Genes, Viral, Hela Cells/metabolism, Humans, Plasmids, Promoter Regions, Genetic, Protein Kinases, Proto-Oncogene Proteins c-jun, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins, Simian virus 40/genetics, Species Specificity, Transcription Factors/analysis, Transcription Factors/genetics, Transcription, Genetic
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