Automated donor tissue preparation for descemet membrane automated endothelial keratoplasty (DMAEK): an experimental study.
Details
Serval ID
serval:BIB_44DA82321F57
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Automated donor tissue preparation for descemet membrane automated endothelial keratoplasty (DMAEK): an experimental study.
Journal
Ophthalmic surgery, lasers & imaging
ISSN
1938-2375 (Electronic)
ISSN-L
1542-8877
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
42
Number
2
Pages
158-161
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Graft harvesting for Descemet membrane endothelial keratoplasty (DMEK) occurs manually and demonstrates surgical difficulties. In the current study, a mechanical automated technique for preparing a donor Descemet membrane (DM) carrying autologous endothelium for DMEK was evaluated.
Ten rabbit corneas were placed on an artificial anterior chamber and mechanical separation of the DM was conducted using an Epi-keratome (Senturium; Norwood Abbey EyeCare, Victoria, Australia). All DM specimens were properly fixated and optical microscopy was performed.
DM separation was achieved in a controlled fashion in 7 of 10 eyes. As demonstrated by optical microscopy of the specimens, no corneal stroma was attached on the DM, whereas the endothelial layer was preserved in several areas.
Automated separation of the DM can be achieved using an Epi-keratome. Additional studies and improvements of the current technique are needed to draw final conclusions.
Ten rabbit corneas were placed on an artificial anterior chamber and mechanical separation of the DM was conducted using an Epi-keratome (Senturium; Norwood Abbey EyeCare, Victoria, Australia). All DM specimens were properly fixated and optical microscopy was performed.
DM separation was achieved in a controlled fashion in 7 of 10 eyes. As demonstrated by optical microscopy of the specimens, no corneal stroma was attached on the DM, whereas the endothelial layer was preserved in several areas.
Automated separation of the DM can be achieved using an Epi-keratome. Additional studies and improvements of the current technique are needed to draw final conclusions.
Keywords
Animals, Automation, Descemet Membrane/surgery, Descemet Stripping Endothelial Keratoplasty, Endothelium, Corneal/surgery, Endothelium, Corneal/transplantation, Microscopy/methods, Rabbits, Tissue Donors, Tissue and Organ Harvesting/methods, Tissue and Organ Harvesting/standards
Pubmed
Web of science
Create date
01/10/2019 16:10
Last modification date
06/10/2019 6:26
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