Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized (13) C-Labeled Molecular Probe.
Details
Serval ID
serval:BIB_43E53D072042
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized (13) C-Labeled Molecular Probe.
Journal
Angewandte Chemie
ISSN
1521-3773 (Electronic)
ISSN-L
1433-7851
Publication state
Published
Issued date
26/08/2016
Peer-reviewed
Oui
Volume
55
Number
36
Pages
10626-10629
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-(13) C]Gly, for monitoring GGT activity in vivo by hyperpolarized (HP) (13) C magnetic resonance (MR). The properties of γ-Glu-[1-(13) C]Gly are suitable for in vivo HP (13) C metabolic analysis since the chemical shift between γ-Glu-[1-(13) C]Gly and its metabolic product, [1-(13) C]Gly, is large (4.3 ppm) and the T1 of both compounds is relatively long (30 s and 45 s, respectively, in H2 O at 9.4 T). We also demonstrate that γ-Glu-[1-(13) C]Gly is highly sensitive to in vivo modulation of GGT activity induced by the inhibitor acivicin.
Keywords
Animals, Enzyme Assays/methods, Enzyme Inhibitors/pharmacology, Glutathione/metabolism, Isoxazoles/pharmacology, Molecular Probes/metabolism, Nuclear Magnetic Resonance, Biomolecular/methods, Rats, gamma-Glutamyltransferase/antagonists & inhibitors, gamma-Glutamyltransferase/metabolism, NMR spectroscopy, biosensors, dynamic nuclear polarization, hyperpolarization, γ-glutamyl transpeptidase
Pubmed
Web of science
Open Access
Yes
Create date
11/01/2018 10:29
Last modification date
20/08/2019 14:48
Usage data
