CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.

Détails

ID Serval
serval:BIB_43BAB52D4E39
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.
Périodique
Molecular and Cellular Biology
Auteur(s)
Yuan C.C., Zhao X., Florens L., Swanson S.K., Washburn M.P., Hernandez N.
ISSN
1098-5549[electronic]
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
27
Numéro
24
Pages
8729-8738
Langue
anglais
Résumé
Chromatin remodeling and histone modification are essential for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (Pol III)-transcribed genes. The human U6 small nuclear RNA promoter, located 5' of the transcription start site, consists of a core region directing basal transcription and an activating region that recruits the transcription factors Oct-1 and Staf (ZNF143). Oct-1 activates transcription in part by helping recruit core binding factors, but nothing is known about the mechanisms of transcription activation by Staf. We show that Staf activates U6 transcription from a preassembled chromatin template in vitro and associates with several proteins linked to chromatin modification, among them chromodomain-helicase-DNA binding protein 8 (CHD8). CHD8 binds to histone H3 di- and trimethylated on lysine 4. It resides on the human U6 promoter as well as the mRNA IRF3 promoter in vivo and contributes to efficient transcription from both these promoters. Thus, Pol III transcription from type 3 promoters uses some of the same factors used for chromatin remodeling at Pol II promoters.
Mots-clé
Amino Acid Sequence, Chromatin/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/metabolism, Hela Cells, Histones/metabolism, Humans, Immunoprecipitation, Interferon Regulatory Factor-3/metabolism, Lysine/metabolism, Methylation, Molecular Sequence Data, Protein Binding, Protein Transport, RNA Polymerase III, RNA, Small Nuclear/genetics, RNA, Small Nuclear/metabolism, Trans-Activators/metabolism, Transcription Factors/chemistry, Transcription Factors/metabolism, Transcription, Genetic, Transcriptional Activation/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 14:47
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