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Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.
The microenvironment hosting a tumor actively participates in regulating tumor cell proliferation, migration, and invasion. Among the extracellular matrix proteins enriched in the stroma of carcinomas are the tenascin family members tenascin-C and tenascin-W. Whereas tenascin-C overexpression in gliomas is known to correlate with poor prognosis, the status of tenascin-W in brain tumors has not been investigated so far. In the present study, we analyzed protein levels of tenascin-W in 38 human gliomas and found expression of tenascin-W in 80% of the tumor samples, whereas no tenascin-W could be detected in control, nontumoral brain tissues. Double immunohistochemical staining of tenascin-W and von Willebrand factor revealed that tenascin-W is localized around blood vessels, exclusively in tumor samples. In vitro, the presence of tenascin-W increased the proportion of elongated human umbilical vein endothelial cells (HUVECs) and augmented the mean speed of cell migration. Furthermore, tenascin-W triggered sprouting of HUVEC spheroids to a similar extent as the proangiogenic factor tenascin-C. In conclusion, our study identifies tenascin-W as a candidate biomarker for brain tumor angiogenesis that could be used as a molecular target for therapy irrespective of the glioma subtype.-Martina, E., Degen, M., Rüegg, C., Merlo, A., Lino, M. M., Chiquet-Ehrismann, R., Brellier, F. Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.
Blood Vessels/metabolism, Blotting, Western, Cell Enlargement/drug effects, Cell Line, Cell Movement/drug effects, Cells, Cultured, Endothelial Cells/cytology, Endothelial Cells/drug effects, Fluorescent Antibody Technique, Glioma/metabolism, Humans, Neovascularization, Physiologic/drug effects, Spheroids, Cellular/drug effects, Tenascin/metabolism, Tenascin/pharmacology, Tumor Markers, Biological/metabolism, Tumor Markers, Biological/pharmacology, Umbilical Veins/cytology
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