In vivo 13C NMR assessment of brain glycogen concentration and turnover in the awake rat.

Details

Serval ID
serval:BIB_4338847DA74D
Type
Article: article from journal or magazin.
Collection
Publications
Title
In vivo 13C NMR assessment of brain glycogen concentration and turnover in the awake rat.
Journal
Neurochemistry International
Author(s)
Choi I.Y., Gruetter R.
ISSN
0197-0186 (Print)
ISSN-L
0197-0186
Publication state
Published
Issued date
2003
Volume
43
Number
4-5
Pages
317-322
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Brain glycogen metabolism was recently observed in vivo and found to be very slow in the lightly alpha-chloralose anesthetized rat [J. Neurochem. 73 (1999) 1300]. Based on that slow turnover, the total glycogen content in the awake rat brain and its turnover time were assessed after administering 13C-labeled glucose for 48 h. Label incorporation into glycogen, glucose, amino acid, and N-acetyl-aspartate (NAA) resonances was observed. The amount of 13C label incorporated into glycogen was variable and did not correlate with that in glutamate (r=-0.1, P>0.86). However, the amount of 13C label incorporated into glycogen was very similar to that in NAA (r=0.93), implying similar turnover times between brain glycogen and NAA (approximately 10 h). Absolute quantification of the total concentration of brain glycogen in the awake, normoglycemic rat yielded 3.3+/-0.8 micromol/g (n=6, mean+/-S.D.).
Keywords
Animals, Brain/metabolism, Carbon Isotopes, Glycogen/metabolism, Magnetic Resonance Spectroscopy, Male, Rats, Rats, Sprague-Dawley, Wakefulness
Pubmed
Web of science
Create date
04/08/2010 15:28
Last modification date
20/08/2019 13:46
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