In vivo 13C NMR assessment of brain glycogen concentration and turnover in the awake rat.
Details
Serval ID
serval:BIB_4338847DA74D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vivo 13C NMR assessment of brain glycogen concentration and turnover in the awake rat.
Journal
Neurochemistry International
ISSN
0197-0186 (Print)
ISSN-L
0197-0186
Publication state
Published
Issued date
2003
Volume
43
Number
4-5
Pages
317-322
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Brain glycogen metabolism was recently observed in vivo and found to be very slow in the lightly alpha-chloralose anesthetized rat [J. Neurochem. 73 (1999) 1300]. Based on that slow turnover, the total glycogen content in the awake rat brain and its turnover time were assessed after administering 13C-labeled glucose for 48 h. Label incorporation into glycogen, glucose, amino acid, and N-acetyl-aspartate (NAA) resonances was observed. The amount of 13C label incorporated into glycogen was variable and did not correlate with that in glutamate (r=-0.1, P>0.86). However, the amount of 13C label incorporated into glycogen was very similar to that in NAA (r=0.93), implying similar turnover times between brain glycogen and NAA (approximately 10 h). Absolute quantification of the total concentration of brain glycogen in the awake, normoglycemic rat yielded 3.3+/-0.8 micromol/g (n=6, mean+/-S.D.).
Keywords
Animals, Brain/metabolism, Carbon Isotopes, Glycogen/metabolism, Magnetic Resonance Spectroscopy, Male, Rats, Rats, Sprague-Dawley, Wakefulness
Pubmed
Web of science
Create date
04/08/2010 16:28
Last modification date
20/08/2019 14:46