Agonist-induced desensitization of dopamine D-1 receptors in bovine retina and rat striatum

Détails

ID Serval
serval:BIB_431A7DDFC830
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Agonist-induced desensitization of dopamine D-1 receptors in bovine retina and rat striatum
Périodique
Journal of Pharmacology and Experimental Therapeutics
Auteur(s)
Ofori  S., Bugnon  O., Schorderet  M.
ISSN
0022-3565 (Print)
Statut éditorial
Publié
Date de publication
07/1993
Volume
266
Numéro
1
Pages
350-7
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
We have investigated agonist-induced desensitization of dopamine (DA) D-1 receptor mediated accumulation of adenosine 3':5'-cyclic monophosphate (cAMP) in bovine retinas and rat striata in vitro. Tissues were exposed to various pharmacological agents for 60 min, washed and homogenized. D-1 agonist-promoted accumulation of cAMP in the presence of adenosine 5'-triphosphate (ATP) and 5'-guanylylimidodiphosphate (GppNHp) was then measured. Retinas and striata incubated with DA or (+) SKF 38393 (100 microM each) displayed diminished capacities to accumulate cAMP when they were re-exposed to the same drugs. As expected, retinas and striata treated wtih (+) SKF 38393 also showed an attenuated formation of cAMP when restimulated with DA. In addition, D-1 receptors in retinas that had been treated with quinpirole, a D-2 agonist, did not become desensitized. Furthermore, 0.2 microM SCH 23390 prevented D-1 receptor subsensitivity caused by 100 microM DA. Interestingly, retinas and striata incubated with forskolin, a direct stimulator of adenylyl cyclase, also displayed a diminished enzymatic response to restimulation by the same agent, as well as by DA. Additionally, retinas incubated with 100 microM DA were refractory to forskolin-induced cAMP formation. Finally, results of 3H-SCH 23390 binding to control and DA-treated retinas indicated that agonist treatment did not induce significant changes in either receptor density or binding affinity. The data indicate that bovine retinas and striatal slices in vitro are suitable to investigate various aspects of the desensitization of neuronal responses to DA D-1 agonists or forskolin.
Mots-clé
Adenylate Cyclase/metabolism Animals Cattle Corpus Striatum/drug effects/metabolism/*ultrastructure Cyclic AMP/biosynthesis Forskolin/pharmacology Rats Receptors, Dopamine D1/drug effects/metabolism/*physiology Retina/drug effects/metabolism/*ultrastructure Sensitivity and Specificity Stimulation, Chemical
Pubmed
Web of science
Création de la notice
28/01/2008 12:42
Dernière modification de la notice
03/03/2018 16:40
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