CD4(+) T-cell- and gamma interferon-dependent protection against murine malaria by immunization with linear synthetic peptides from a Plasmodium yoelii 17-kilodalton hepatocyte erythrocyte protein

Détails

ID Serval
serval:BIB_42D44A62CC5F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CD4(+) T-cell- and gamma interferon-dependent protection against murine malaria by immunization with linear synthetic peptides from a Plasmodium yoelii 17-kilodalton hepatocyte erythrocyte protein
Périodique
Infection and Immunity
Auteur(s)
Charoenvit  Y., Majam  V. F., Corradin  G., Sacci, J. B., Jr. , Wang  R., Doolan  D. L., Jones  T. R., Abot  E., Patarroyo  M. E., Guzman  F., Hoffman  S. L.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
11/1999
Volume
67
Numéro
11
Pages
5604-14
Notes
Journal Article
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Nov
Résumé
Most work on protective immunity against the pre-erythrocytic stages of malaria has focused on induction of antibodies that prevent sporozoite invasion of hepatocytes, and CD8(+) T-cell responses that eliminate infected hepatocytes. We recently reported that immunization of A/J mice with an 18-amino-acid synthetic linear peptide from Plasmodium yoelii sporozoite surface protein 2 (SSP2) in TiterMax adjuvant induces sterile protection that is dependent on CD4(+) T cells and gamma interferon (IFN-gamma). We now report that immunization of inbred A/J mice and outbred CD1 mice with each of two linear synthetic peptides from the 17-kDa P. yoelii hepatocyte erythrocyte protein (HEP17) in the same adjuvant also induces protection against sporozoite challenge that is dependent on CD4(+) T cells and IFN-gamma. The SSP2 peptide and the two HEP17 peptides are recognized by B cells as well as T cells, and the protection induced by these peptides appears to be directed against the infected hepatocytes. In contrast to the peptide-induced protection, immunization of eight different strains of mice with radiation-attenuated sporozoites induces protection that is absolutely dependent on CD8(+) T cells. Data represented here demonstrate that CD4(+) T-cell-dependent protection can be induced by immunization with linear synthetic peptides. These studies therefore provide the foundation for an approach to pre-erythrocytic-stage malaria vaccine development, based on the induction of protective CD4(+) T-cell responses, which will complement efforts to induce protective antibody and CD8(+) T-cell responses.
Mots-clé
Amino Acid Sequence Animals Antibodies, Protozoan/blood Antigens, Protozoan/*immunology CD4-Positive T-Lymphocytes/*immunology Epitope Mapping Female Immunization Immunoglobulin Isotypes/blood Interferon Type II/*physiology Malaria/*prevention & control Malaria Vaccines/*immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Plasmodium yoelii/*immunology Protozoan Proteins/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
03/03/2018 16:39
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