Reticulon 1-C/neuroendocrine-specific protein-C interacts with SNARE proteins

Détails

ID Serval
serval:BIB_42C6481D31F9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Reticulon 1-C/neuroendocrine-specific protein-C interacts with SNARE proteins
Périodique
Journal of Neurochemistry
Auteur(s)
Steiner  P., Kulangara  K., Sarria  J. C., Glauser  L., Regazzi  R., Hirling  H.
ISSN
0022-3042 (Print)
Statut éditorial
Publié
Date de publication
05/2004
Volume
89
Numéro
3
Pages
569-80
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Reticulons are proteins of neuroendocrine cells localized primarily to the endoplasmic reticulum membrane. Despite their implication in cellular processes like apoptosis or axonal regeneration, their intracellular molecular function is still largely unknown. Here, we show that reticulon 1-C can be detected in a protein complex of 150-200 kDa, and that a number of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, i.e. syntaxin 1, syntaxin 7, syntaxin 13 and VAMP2, can be co-immunoprecipitated with reticulon 1-C. Moreover, it localizes to a nocodazole-sensitive, but calreticulin-negative domain of the endoplasmic reticulum. Finally, overexpression in PC12 cells of a reticulon 1-C fragment which binds to SNAREs, significantly enhances human growth hormone secretion. These results suggest that reticulons are involved in vesicle trafficking events, including regulated exocytosis.
Mots-clé
Animals Antigens, Surface/metabolism Cells, Cultured Endoplasmic Reticulum/drug effects/metabolism Exocytosis/physiology Human Growth Hormone Humans Immune Sera/metabolism Macromolecular Substances Membrane Proteins/*metabolism Nerve Tissue Proteins/*metabolism Neurons/cytology/*metabolism PC12 Cells Precipitin Tests Protein Binding/physiology Protein Transport/physiology Qa-SNARE Proteins R-SNARE Proteins Rats SNARE Proteins Syntaxin 1 *Vesicular Transport Proteins
Pubmed
Web of science
Création de la notice
24/01/2008 15:30
Dernière modification de la notice
03/03/2018 16:38
Données d'usage