Multiplex quantitative imaging of human myocardial infarction by mass spectrometry-immunohistochemistry.

Détails

ID Serval
serval:BIB_42921203C5A3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Multiplex quantitative imaging of human myocardial infarction by mass spectrometry-immunohistochemistry.
Périodique
International journal of legal medicine
Auteur(s)
Aljakna A., Lauer E., Lenglet S., Grabherr S., Fracasso T., Augsburger M., Sabatasso S., Thomas A.
ISSN
1437-1596 (Electronic)
ISSN-L
0937-9827
Statut éditorial
Publié
Date de publication
11/2018
Peer-reviewed
Oui
Volume
132
Numéro
6
Pages
1675-1684
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Simultaneous assessment of a panel of protein markers is becoming essential in order to enhance biomarker research and improve diagnostics. Specifically, postmortem diagnostics of early myocardial ischemia in sudden cardiac death cases could benefit from a multiplex marker assessment in the same tissue section. Current analytical antibody-based techniques (immunohistochemistry and immunofluorescence) limit multiplex analysis usually to not more than three antibodies. In this study, mass spectrometry-immunohistochemistry (MS-IHC) was performed by combining laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) with rare-metal-isotope-tagged antibodies as a technique for multiplex analysis of human postmortem myocardial tissue samples. Tissue sections with myocardial infarction were simultaneously analyzed for seven primary, rare-metal-isotope-tagged antibodies (troponin T, myoglobin, fibronectin, C5b-9, unphosphorylated connexin 43, VEGF-B, and JunB). Comparison between the MS-IHC approach and chromogenic IHC showed similar patterns in ionic and optical images. In addition, absolute quantification was performed by MS-IHC, providing a proportional relationship between the signal intensity and the local marker concentration in tissue sections. These data demonstrated that LA-ICP-MS combined with rare-metal-isotope-tagged antibodies is an efficient strategy for simultaneous testing of multiple markers and allows not only visualization of molecules within the tissue but also quantification of the signal. Such imaging approach has a great potential in both diagnostics and pathology-related research.
Mots-clé
Biomarkers/metabolism, Complement Membrane Attack Complex/metabolism, Connexin 43/metabolism, Female, Forensic Pathology, Humans, Immunohistochemistry, Isotopes, Male, Mass Spectrometry/methods, Middle Aged, Myocardial Infarction/metabolism, Myocardium/metabolism, Myocardium/pathology, Myocytes, Cardiac/metabolism, Myoglobin, Transcription Factors, Troponin T/metabolism, Vascular Endothelial Growth Factor B, Biomarker, Forensic pathology, Mass spectrometry-immunohistochemistry, Multiplex tissue imaging, Myocardial ischemia
Pubmed
Web of science
Création de la notice
21/03/2018 18:19
Dernière modification de la notice
27/11/2018 7:26
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