An integrated genetic and functional analysis of the role of type II transmembrane serine proteases (TMPRSSs) in hearing loss.

Détails

ID Serval
serval:BIB_427430949C56
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An integrated genetic and functional analysis of the role of type II transmembrane serine proteases (TMPRSSs) in hearing loss.
Périodique
Human Mutation
Auteur(s)
Guipponi M., Toh M.Y., Tan J., Park D., Hanson K., Ballana E., Kwong D., Cannon P.Z., Wu Q., Gout A., Delorenzi M., Speed T.P., Smith R.J., Dahl H.H., Petersen M., Teasdale R.D., Estivill X., Park W.J., Scott H.S.
ISSN
1098-1004 (Electronic)
ISSN-L
1059-7794
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
29
Numéro
1
Pages
130-141
Langue
anglais
Résumé
Building on our discovery that mutations in the transmembrane serine protease, TMPRSS3, cause nonsyndromic deafness, we have investigated the contribution of other TMPRSS family members to the auditory function. To identify which of the 16 known TMPRSS genes had a strong likelihood of involvement in hearing function, three types of biological evidence were examined: 1) expression in inner ear tissues; 2) location in a genomic interval that contains a yet unidentified gene for deafness; and 3) evaluation of hearing status of any available Tmprss knockout mouse strains. This analysis demonstrated that, besides TMPRSS3, another TMPRSS gene was essential for hearing and, indeed, mice deficient for Hepsin (Hpn) also known as Tmprss1 exhibited profound hearing loss. In addition, TMPRSS2, TMPRSS5, and CORIN, also named TMPRSS10, showed strong likelihood of involvement based on their inner ear expression and mapping position within deafness loci PKSR7, DFNB24, and DFNB25, respectively. These four TMPRSS genes were then screened for mutations in affected members of the DFNB24 and DFNB25 deafness families, and in a cohort of 362 sporadic deaf cases. This large mutation screen revealed numerous novel sequence variations including three potential pathogenic mutations in the TMPRSS5 gene. The mutant forms of TMPRSS5 showed reduced or absent proteolytic activity. Subsequently, TMPRSS genes with evidence of involvement in deafness were further characterized, and their sites of expression were determined. Tmprss1, 3, and 5 proteins were detected in spiral ganglion neurons. Tmprss3 was also present in the organ of Corti. TMPRSS1 and 3 proteins appeared stably anchored to the endoplasmic reticulum membranes, whereas TMPRSS5 was also detected at the plasma membrane. Collectively, these results provide evidence that TMPRSS1 and TMPRSS3 play and TMPRSS5 may play important and specific roles in hearing.
Mots-clé
Animals, Ear, Inner/metabolism, Hearing Loss/enzymology, Hearing Loss/genetics, Humans, Immunohistochemistry, Membrane Proteins/analysis, Membrane Proteins/genetics, Mice, Mice, Knockout, Mutation, Neoplasm Proteins/genetics, Neoplasm Proteins/metabolism, Serine Endopeptidases/analysis, Serine Endopeptidases/genetics
Pubmed
Web of science
Création de la notice
23/02/2012 13:36
Dernière modification de la notice
03/03/2018 16:37
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