A minimal RNA polymerase III transcription system from human cells reveals positive and negative regulatory roles for CK2.

Détails

ID Serval
serval:BIB_4244B4D8BFB9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A minimal RNA polymerase III transcription system from human cells reveals positive and negative regulatory roles for CK2.
Périodique
Molecular Cell
Auteur(s)
Hu P., Wu S., Hernandez N.
ISSN
1097-2765[print], 1097-2765[linking]
Statut éditorial
Publié
Date de publication
2003
Volume
12
Numéro
3
Pages
699-709
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
In higher eukaryotes, RNA polymerase (pol) III is known to use different transcription factors to recognize three basic types of promoters, but in no case have these transcription factors been completely defined. We show that a highly purified pol III complex combined with the recombinant transcription factors SNAP(c), TBP, Brf2, and Bdp1 directs multiple rounds of transcription initiation and termination from the human U6 promoter. The pol III complex contains traces of CK2, and CK2 associates with the U6 promoter region in vivo. Transcription requires CK2 phosphorylation of the pol III complex. In contrast, CK2 phosphorylation of TBP, Brf2, and Bdp1 combined is inhibitory. The results define a minimum core machinery, the ultimate target of regulatory mechanisms, capable of directing all steps of the transcription process-initiation, elongation, and termination-by a metazoan RNA polymerase, and suggest positive and negative regulatory roles for CK2 in transcription by pol III.
Mots-clé
Animals, Casein Kinase II, Cell-Free System, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Eukaryotic Cells/enzymology, Feedback, Physiological/genetics, Genes, Regulator/genetics, Hela Cells, Humans, Phosphorylation, Promoter Regions, Genetic/genetics, Proteasome Endopeptidase Complex, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, RNA Polymerase III/genetics, RNA Polymerase III/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Transcription Factor TFIIIB/genetics, Transcription Factor TFIIIB/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcription, Genetic/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 14:44
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