Localized in vivo 13C NMR spectroscopy of the brain.

Details

Serval ID
serval:BIB_4225356D09EF
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Localized in vivo 13C NMR spectroscopy of the brain.
Journal
NMR in Biomedicine
Author(s)
Gruetter R., Adriany G., Choi I.Y., Henry P.G., Lei H., Oz G.
ISSN
0952-3480 (Print)
ISSN-L
0952-3480
Publication state
Published
Issued date
2003
Volume
16
Number
6-7
Pages
313-338
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. ; ReviewPublication Status: ppublish
Abstract
Localized (13)C NMR spectroscopy provides a new investigative tool for studying cerebral metabolism. The application of (13)C NMR spectroscopy to living intact humans and animals presents the investigator with a number of unique challenges. This review provides in the first part a tutorial insight into the ingredients required for achieving a successful implementation of localized (13)C NMR spectroscopy. The difficulties in establishing (13)C NMR are the need for decoupling of the one-bond (13)C-(1)H heteronuclear J coupling, the large chemical shift range, the low sensitivity and the need for localization of the signals. The methodological consequences of these technical problems are discussed, particularly with respect to (a) RF front-end considerations, (b) localization methods, (c) the low sensitivity, and (d) quantification methods. Lastly, some achievements of in vivo localized (13)C NMR spectroscopy of the brain are reviewed, such as: (a) the measurement of brain glutamine synthesis and the feasibility of quantifying glutamatergic action in the brain; (b) the demonstration of significant anaplerotic fluxes in the brain; (c) the demonstration of a highly regulated malate-aspartate shuttle in brain energy metabolism and isotope flux; (d) quantification of neuronal and glial energy metabolism; and (e) brain glycogen metabolism in hypoglycemia in rats and humans. We conclude that the unique and novel insights provided by (13)C NMR spectroscopy have opened many new research areas that are likely to improve the understanding of brain carbohydrate metabolism in health and disease.
Keywords
Animals, Brain/metabolism, Carbon Isotopes/diagnostic use, Glucose/metabolism, Glutamic Acid/metabolism, Glycogen/metabolism, Humans, Magnetic Resonance Spectroscopy/instrumentation, Magnetic Resonance Spectroscopy/methods, Neurons/metabolism, Neurotransmitter Agents/metabolism, Staining and Labeling/methods, Tissue Distribution
Pubmed
Web of science
Open Access
Yes
Create date
04/08/2010 15:28
Last modification date
20/08/2019 13:43
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