CpG oligodeoxynucleotides activate dendritic cells in vivo and induce a functional and protective vaccine immunity against a TERT derived modified cryptic MHC class I-restricted epitope
Details
Serval ID
serval:BIB_4197B019D7E6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CpG oligodeoxynucleotides activate dendritic cells in vivo and induce a functional and protective vaccine immunity against a TERT derived modified cryptic MHC class I-restricted epitope
Journal
Vaccine
ISSN
0264-410X (Print)
Publication state
Published
Issued date
03/2006
Volume
24
Number
11
Pages
1880-8
Notes
Comparative Study
Journal Article --- Old month value: Mar 10
Journal Article --- Old month value: Mar 10
Abstract
The use of synthetic peptides derived from tumor-associated Ags is attractive for the development of antitumoral vaccines as far as strong adjuvants are found to render them immunogenic. Here, we investigated the possibility to enhance the CD8 response against the human and mouse shared TERT(572Y) HLA-A*0201 restricted modified cryptic peptide by using ODN-CpG as adjuvant. Humanized transgenic mice were immunized with the TERT(572Y) modified cryptic peptide in the presence of ODN-CpG and compared to mice immunized in IFA. By contrast with IFA, we first showed that, in vivo, ODN-CpG leads to the recruitment of dendritic cells in the lymph nodes draining the injection site. Those cells and especially the CD11c+ CD11b- CD8a+ lymphoid and the CD11c+ B220+ plasmacytoid dendritic cells were activated as shown by up-regulation of CD40 at their cell surface. Immunization against TERT(572Y) peptide in the presence of ODN-CpG rather than IFA led to a strong CD8 response and can delayed mortality in an induced tumor model. Study of the CD8 response obtained after antigenic challenge suggested that a functional memory response is induced upon vaccination with ODN-CpG. Thus, MHC class I-restricted epitope in combination of ODN-CpG is a promising and rather simple cancer vaccine formulation.
Keywords
Adjuvants, Immunologic/administration & dosage
Animals
Antigen-Presenting Cells
Antigens, CD40/analysis
CD8-Positive T-Lymphocytes/immunology
Cancer Vaccines/administration & dosage/*immunology
DNA-Binding Proteins/*immunology
Dendritic Cells/*immunology
Epitopes
Epitopes, T-Lymphocyte/*immunology
Flow Cytometry
Freund's Adjuvant/administration & dosage
HLA-A Antigens/immunology
Humans
Interferon Type II/biosynthesis
Lipids/administration & dosage
Lymph Nodes/cytology
Mice
Mice, Transgenic
Neoplasms, Experimental/*prevention & control
Oligodeoxyribonucleotides/administration & dosage/*immunology
Survival Analysis
Telomerase/*immunology
Pubmed
Web of science
Create date
25/01/2008 16:08
Last modification date
20/08/2019 14:42