Single cell analysis reveals similar functional competence of dominant and nondominant CD8 T-cell clonotypes.

Détails

Ressource 1Télécharger: BIB_4190E280E4E6.P001.pdf (5632.32 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_4190E280E4E6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Single cell analysis reveals similar functional competence of dominant and nondominant CD8 T-cell clonotypes.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Speiser D.E., Wieckowski S., Gupta B., Iancu E.M., Baumgaertner P., Baitsch L., Michielin O., Romero P., Rufer N.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2011
Volume
108
Numéro
37
Pages
15318-15323
Langue
anglais
Notes
Publication types: Clinical Trial, Phase I ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Immune protection from infectious diseases and cancer is mediated by individual T cells of different clonal origin. Their functions are tightly regulated but not yet fully characterized. Understanding the contribution of each T cell will improve the prediction of immune protection based on laboratory assessment of T-cell responses. Here we developed techniques for simultaneous molecular and functional assessment of single CD8 T cells directly ex vivo. We studied two groups of patients with melanoma after vaccination with two closely related tumor antigenic peptides. Vaccination induced T cells with strong memory and effector functions, as found in virtually all T cells of the first patient group, and fractions of T cells in the second group. Interestingly, high functionality was not restricted to dominant clonotypes. Rather, dominant and nondominant clonotypes acquired equal functional competence. In parallel, this was also found for EBV- and CMV-specific T cells. Thus, the nondominant clonotypes may contribute similarly to immunity as their dominant counterparts.
Mots-clé
Amino Acid Sequence, Antigens, Neoplasm/immunology, CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, Cancer Vaccines/immunology, Cell Differentiation/immunology, Cell Proliferation, Clone Cells, Cytomegalovirus/immunology, Cytotoxicity, Immunologic, Gene Expression Profiling, Humans, Melanoma/immunology, Molecular Sequence Data, Receptors, Antigen, T-Cell/immunology, Single-Cell Analysis/methods, Species Specificity, Vaccination, Vaccines, Subunit/chemistry, Vaccines, Subunit/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/01/2012 16:43
Dernière modification de la notice
08/05/2019 17:41
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