Sonication of catheter tips for improved detection of microorganisms on external ventricular drains and ventriculo-peritoneal shunts.

Details

Serval ID
serval:BIB_4189822F44AB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sonication of catheter tips for improved detection of microorganisms on external ventricular drains and ventriculo-peritoneal shunts.
Journal
Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia
Author(s)
Jost G.F., Wasner M., Taub E., Walti L., Mariani L., Trampuz A.
ISSN
1532-2653 (Electronic)
ISSN-L
0967-5868
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
21
Number
4
Pages
578-582
Language
english
Notes
Publication types: Journal Article, pdf : clinical study
Abstract
The diagnosis of infections involving internal or external neurosurgical drainage devices is challenging, and to our knowledge no single reliable microbiological test exists. We used sonication to study bacterial colonization in 14 explanted external ventricular drains (EVD) and 13 ventriculo-peritoneal shunt (VPS) devices. This technique dislodges biofilm bacteria from the surface of implanted materials before culture. Removed devices were sonicated in saline (40 kHz, 1 minute, 0.25 W/cm(2)), the resulting fluid was cultured aerobically and anaerobically at 37°C, and bacterial growth was counted. Ventricular cerebrospinal fluid (CSF) was cultured separately. In the EVD group, sonication cultures grew significantly more bacteria (64%, 9/14) than cultures of aspirated ventricular CSF (14%, 2/14). In the VPS group the difference was not significant. Positive sonication cultures of EVD catheters yielded a median of >100 colony forming units (CFU) (range, 60-800). For positive sonication cultures of VPS, the median was 1000 CFU (range, 20-100,000). All patients with bacteria in their CSF also had positive sonication cultures from the removed device. Of the five patients with sterile or presumably contaminated CSF cultures but positive sonication cultures of removed shunts, one became afebrile after removal of the EVD, two developed meningitis and two remained asymptomatic. Sonication culture of EVD appears to improve the microbiological assessment of device-related infection and it corroborates with CSF cultures of revision surgery for VPS. Sonication of the removed EVD tip may raise awareness for the onset of meningitis.
Pubmed
Web of science
Create date
11/05/2014 14:34
Last modification date
20/08/2019 13:42
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