CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in <i>Arabidopsis</i>.

Détails

Ressource 1Télécharger: dev162354.full.pdf (2000.23 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_41871942560A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in <i>Arabidopsis</i>.
Périodique
Development
Auteur(s)
Anne P., Amiguet-Vercher A., Brandt B., Kalmbach L., Geldner N., Hothorn M., Hardtke C.S.
ISSN
1477-9129 (Electronic)
ISSN-L
0950-1991
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
145
Numéro
10
Pages
NA
Langue
anglais
Résumé
CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are secreted endogenous plant ligands that are sensed by receptor kinases (RKs) to convey environmental and developmental inputs. Typically, this involves an RK with narrow ligand specificity that signals together with a more promiscuous co-receptor. For most CLEs, biologically relevant (co-)receptors are unknown. The dimer of the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) conditions perception of so-called root-active CLE peptides, the exogenous application of which suppresses root growth by preventing protophloem formation in the meristem. <i>clv2</i> as well as <i>crn</i> null mutants are resistant to root-active CLE peptides, possibly because CLV2-CRN promotes expression of their cognate receptors. Here, we have identified the <i>CLE-RESISTANT RECEPTOR KINASE</i> ( <i>CLERK</i> ) gene, which is required for full sensing of root-active CLE peptides in early developing protophloem. CLERK protein can be replaced by its close homologs, SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK) and NSP-INTERACTING KINASE 1 (NIK1). Yet neither CLERK nor NIK1 ectodomains interact biochemically with described CLE receptor ectodomains. Consistently, <i>CLERK</i> also acts genetically independently of <i>CLV2-CRN</i> We, thus, have discovered a novel hub for redundant CLE sensing in the root.
Mots-clé
Arabidopsis/enzymology, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Genome, Plant/genetics, Membrane Proteins/genetics, Plant Roots/enzymology, Plants, Genetically Modified, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, Arabidopsis, BAM3, CLE peptide, Protophloem, Receptor kinase, Root
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/05/2018 17:26
Dernière modification de la notice
08/05/2019 17:40
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