Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model.

Détails

ID Serval
serval:BIB_416DCA6C7AC6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model.
Périodique
Cellular Microbiology
Auteur(s)
Alibaud L., Köhler T., Coudray A., Prigent-Combaret C., Bergeret E., Perrin J., Benghezal M., Reimmann C., Gauthier Y., van Delden C., Attree I., Fauvarque M.O., Cosson P.
ISSN
1462-5822[electronic], 1462-5814[linking]
Statut éditorial
Publié
Date de publication
2008
Volume
10
Numéro
3
Pages
729-740
Langue
anglais
Résumé
The human pathogen Pseudomonas aeruginosa has been shown previously to use similar virulence factors when infecting mammalian hosts or Dictyostelium amoebae. Here we randomly mutagenized a clinical isolate of P. aeruginosa, and identified mutants with attenuated virulence towards Dictyostelium. These mutant strains also exhibited a strong decrease in virulence when infecting Drosophila and mice, confirming that P. aeruginosa makes use of similar virulence traits to confront these very different hosts. Further characterization of these bacterial mutants showed that TrpD is important for the induction of the quorum-sensing circuit, while PchH and PchI are involved in the induction of the type III secretion system. These results demonstrate the usefulness and the relevance of the Dictyostelium host model to identify and analyse new virulence genes in P. aeruginosa.
Mots-clé
ATP-Binding Cassette Transporters/genetics, ATP-Binding Cassette Transporters/physiology, Animals, Bacterial Proteins/genetics, Bacterial Proteins/physiology, DNA Transposable Elements, Dictyostelium/microbiology, Drosophila, Female, Humans, Male, Mice, Mutagenesis, Insertional, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/pathogenicity, Survival Analysis, Virulence/genetics, Virulence Factors/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/03/2008 15:39
Dernière modification de la notice
08/05/2019 17:40
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