Article: article from journal or magazin.
Self-assembly of beta-amyloid 42 is retarded by small molecular ligands at the stage of structural intermediates
Journal of Structural Biology
Journal Article --- Old month value: Jun
Assemblyof the amyloid-beta peptide (Abeta) into fibrils and its deposition in distinct brain areas is considered responsible for the pathogenesis of Alzheimer's disease (AD). Thus, inhibition of fibril assembly is a potential strategy for therapeutic intervention. Electron cryomicroscopy was used to monitor the initial, native assembly structure of Abeta42. In addition to the known fibrillar intermediates, a nonfibrillar, polymeric sheet-like structure was identified. A temporary sequence of supramolecular structures was revealed with (i) polymeric Abeta42 sheets during the onset of assembly, inversely related to the appearance of (ii) fibril intermediates, which again are time-dependently replaced by (iii) mature fibrils. A cell-based primary screening assay was used to identify compounds that decrease Abeta42-induced toxicity. Hit compounds were further assayed for binding to Abeta42, radical scavenger activity, and their influence on the assembly structure of Abeta42. One compound, Ro 90-7501, was found to efficiently retard mature fibril formation, while extended polymeric Abeta42 sheets and fibrillar intermediates are accumulated. Ro 90-7501 may serve as a prototypic inhibitor for Abeta42 fibril formation and as a tool for studying the molecular mechanism of fibril assembly.
Amyloid beta-Protein/chemistry/*metabolism/*ultrastructure Benzimidazoles/chemistry/metabolism/pharmacology Cryoelectron Microscopy Free Radical Scavengers/chemistry/metabolism/*pharmacology Humans Ligands Molecular Structure Protein Binding Protein Conformation/drug effects Structure-Activity Relationship Surface Plasmon Resonance Time Factors
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