Replacement of HLA class II serology by the HLA-DR microtitre plate oligotyping assay: a one-year experience in unrelated bone marrow donor selection

Détails

ID Serval
serval:BIB_4128B0A49AB2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Replacement of HLA class II serology by the HLA-DR microtitre plate oligotyping assay: a one-year experience in unrelated bone marrow donor selection
Périodique
British Journal of Haematology
Auteur(s)
Tiercy  J. M., Roosnek  E., Speiser  D., Cros  P., Allibert  P., Mach  B., Jeannet  M.
ISSN
0007-1048 (Print)
Statut éditorial
Publié
Date de publication
10/1993
Volume
85
Numéro
2
Pages
417-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Because the selection of unrelated bone marrow donors requires a more accurate HLA-typing technique than is used for sibling donors, we have replaced class II serology by a rapid and highly discriminative HLA-DR DNA oligotyping assay on microtitre plates. We present here our 1-year experience with class II typing for 112 potential donors identified for 33 consecutive leukaemic patients. Although the donors were selected to be HLA-A, -B, -DR serologically identical, the microtitre plate oligotyping assay detected DR incompatibilities in 52.7% of the patient/donor pairs. One third was due to incorrect or incomplete typing of DR11 to DR16, the others to mismatches for the serologically indistinguishable subtypes of DR1, 11, 13 and 15, known to be relevant for T cell recognition.
Mots-clé
*Bone Marrow Transplantation HLA-D Antigens/blood HLA-DR Antigens/*analysis Histocompatibility Testing/methods Humans Leukemia/surgery Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
28/01/2008 12:33
Dernière modification de la notice
03/03/2018 16:31
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