Characterization of the rice pathogen-related protein Rir1a and regulation of the corresponding gene

Détails

ID Serval
serval:BIB_41219FDFD525
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of the rice pathogen-related protein Rir1a and regulation of the corresponding gene
Périodique
Plant Molecular Biology
Auteur(s)
Mauch  F., Reimmann  C., Freydl  E., Schaffrath  U., Dudler  R.
ISSN
0167-4412 (Print)
Statut éditorial
Publié
Date de publication
11/1998
Volume
38
Numéro
4
Pages
577-86
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 1
Résumé
In rice (Oryza sativa L.), local acquired resistance against Pyricularia oryzae (Cav.), the causal agent of rice blast, can be induced by a preinoculation with the non-host pathogen Pseudomonas syringae pv. syringae. We have cloned a cDNA (Rir1a) and a closely related gene (Rir1b) corresponding to transcripts that accumulate in leaf tissue upon inoculation with P. syringae pv. syringae. The cDNA encodes a putative 107 amino acid protein, Rir1a, that exhibits a putative signal peptide cleavage site in its hydrophobic N-terminal part and a C-terminal part that is relatively rich in glycine and proline. The Rir1b gene contains a Tourist and a Wanderer miniature transposable element in its single intron and encodes a nearly identical protein. Rir1a is similar in sequence (ca. 35% identical and ca. 60% conservatively changed amino acids) to the putative Wir1 family of proteins that are encoded by pathogen-induced transcripts in wheat. Using antibodies raised against a Rir1a-fusion protein we show that Rir1a is secreted from rice protoplasts transiently expressing a 35S::Rir1a construct and that the protein accumulates in the cell wall compartment of rice leaves upon inoculation with P. syringae pv. syringae. Possible roles of Rir1a in pathogen defense are discussed.
Mots-clé
Amino Acid Sequence Ascomycota/pathogenicity Base Sequence Cloning, Molecular DNA Primers/genetics DNA Transposable Elements DNA, Complementary/genetics DNA, Plant/genetics Gene Expression Regulation, Plant *Genes, Plant Molecular Sequence Data Oryza sativa/*genetics/*microbiology/physiology Plant Proteins/chemistry/*genetics/physiology Pseudomonas/pathogenicity RNA, Messenger/genetics/metabolism RNA, Plant/genetics/metabolism Sequence Homology, Amino Acid Triticum/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 15:00
Dernière modification de la notice
03/03/2018 16:31
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