Living autologous heart valves engineered from human prenatally harvested progenitors.

Details

Serval ID
serval:BIB_411AA9D1D7A6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Living autologous heart valves engineered from human prenatally harvested progenitors.
Journal
Circulation
Author(s)
Schmidt D., Mol A., Breymann C., Achermann J., Odermatt B., Gössi M., Neuenschwander S., Prêtre R., Genoni M., Zund G., Hoerstrup S.P.
ISSN
1524-4539 (Electronic)
ISSN-L
0009-7322
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
114
Number
1 Suppl
Pages
I125-I131
Language
english
Abstract
BACKGROUND: Heart valve tissue engineering is a promising strategy to overcome the lack of autologous growing replacements, particularly for the repair of congenital malformations. Here, we present a novel concept using human prenatal progenitor cells as new and exclusive cell source to generate autologous implants ready for use at birth.
METHODS AND RESULTS: Human fetal mesenchymal progenitors were isolated from routinely sampled prenatal chorionic villus specimens and expanded in vitro. A portion was cryopreserved. After phenotyping and genotyping, cells were seeded onto synthetic biodegradable leaflet scaffolds (n=12) and conditioned in a bioreactor. After 21 days, leaflets were endothelialized with umbilical cord blood-derived endothelial progenitor cells and conditioned for additional 7 days. Resulting tissues were analyzed by histology, immunohistochemistry, biochemistry (amounts of extracellular matrix, DNA), mechanical testing, and scanning electron microscopy (SEM) and were compared with native neonatal heart valve leaflets. Fresh and cryopreserved cells showed comparable myofibroblast-like phenotypes. Genotyping confirmed their fetal origin. Neo-tissues exhibited organization, cell phenotypes, extracellular matrix production, and DNA content comparable to their native counterparts. Leaflet surfaces were covered with functional endothelia. SEM showed cellular distribution throughout the polymer and smooth surfaces. Mechanical profiles approximated those of native heart valves.
CONCLUSIONS: Prenatal fetal progenitors obtained from routine chorionic villus sampling were successfully used as an exclusive, new cell source for the engineering of living heart valve leaflets. This concept may enable autologous replacements with growth potential ready for use at birth. Combined with the use of cell banking technology, this approach may be applied also for postnatal applications.
Pubmed
Web of science
Open Access
Yes
Create date
16/12/2014 19:11
Last modification date
20/08/2019 13:40
Usage data