Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion.

Détails

ID Serval
serval:BIB_40E1FE30E6D5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion.
Périodique
Small
Auteur(s)
Li X., Soler M., Szydzik C., Khoshmanesh K., Schmidt J., Coukos G., Mitchell A., Altug H.
ISSN
1613-6829 (Electronic)
ISSN-L
1613-6810
Statut éditorial
Publié
Date de publication
06/2018
Peer-reviewed
Oui
Volume
14
Numéro
26
Pages
e1800698
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Single-cell analysis of cytokine secretion is essential to understand the heterogeneity of cellular functionalities and develop novel therapies for multiple diseases. Unraveling the dynamic secretion process at single-cell resolution reveals the real-time functional status of individual cells. Fluorescent and colorimetric-based methodologies require tedious molecular labeling that brings inevitable interferences with cell integrity and compromises the temporal resolution. An innovative label-free optofluidic nanoplasmonic biosensor is introduced for single-cell analysis in real time. The nanobiosensor incorporates a novel design of a multifunctional microfluidic system with small volume microchamber and regulation channels for reliable monitoring of cytokine secretion from individual cells for hours. Different interleukin-2 secretion profiles are detected and distinguished from single lymphoma cells. The sensor configuration combined with optical spectroscopic imaging further allows us to determine the spatial single-cell secretion fingerprints in real time. This new biosensor system is anticipated to be a powerful tool to characterize single-cell signaling for basic and clinical research.
Mots-clé
cytokine detection, label-free biosensors, microfluidics, nanoplasmonics, real-time single-cell analysis
Pubmed
Web of science
Création de la notice
31/05/2018 18:13
Dernière modification de la notice
16/07/2018 17:21
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