S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi.

Détails

ID Serval
serval:BIB_40C4AA5B9D10
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi.
Périodique
Cytotechnology
Auteur(s)
Grosjean F., Batard P., Jordan M., Wurm F.M.
ISSN
0920-9069 (Print)
ISSN-L
0920-9069
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
38
Numéro
1-3
Pages
57-62
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Various methods exist to transfect mammalian cells in culture. It is generally accepted that individual methods have to be optimized for each of the cell lines or cell types used. Despitethe use of optimized protocols, significant day-to-day variationsin transfection efficiency regularly occur. We postulate that the;status' of cell populations prior to transfection is involved insuch variability. This study evaluates standardized transfectionsdone at different phases of the cell cycle. Cell synchronizationwas achieved using mimosine. Transfection efficiency was monitored by fluorescence quantification of GFP (Green Fluorescent Protein). We show that transfection using the calcium-phosphate-DNA co-precipitation method, at differentphases of the cell cycle, yields variable expression levels of GFP. Highest GFP expression levels were seen when transfecting cell populations with a dominant representation of S-phase-cells.
Pubmed
Web of science
Création de la notice
06/03/2015 13:01
Dernière modification de la notice
03/03/2018 16:29
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