A method for preservation and determination of welding fume nanoparticles in exhaled breath condensate

Détails

Ressource 1Télécharger: 2016_Gschwind_Method_EnvironSciNano_postprint_final.pdf (4283.95 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_40B80D327AE9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A method for preservation and determination of welding fume nanoparticles in exhaled breath condensate
Périodique
Environmental Science: Nano
Auteur(s)
Gschwind Sabrina, Graczyk Halshka, Gunther Detlef, Riediker Michael
ISSN
2051-8153
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Numéro
3
Pages
357-364
Langue
anglais
Résumé
Analysis of exhaled breath condensate (EBC) represents a non-invasive method for detecting inhaled nanoparticles (<100nm; NP) associated with various environmental and occupational exposures. However, the few studies that have investigated inhaled NPs in EBC often assess only bulk, ionic intensities to provide information on overall elemental content, rather than on particulate content. In an attempt to assess inhaled particles in their original particulate form, we developed a methodology for the preservation and determination of inhaled welding fume NPs: a particularly relevant occupational exposure scenario due to the high concentration of metallic NPs produced in the welding process. Two EBC preservation strategies were tested: either flash freezing EBC immediately after collection, or keeping EBC at room temperature until analysis. Particle content of the differently preserved samples was assessed by Microdroplet Generation Inductively Coupled Plasma Mass Spectrometry (MDG-ICP-MS), and with Electron Microscopy. We found that welding fume NPs in EBC may quickly and uncontrollably degrade, thereby losing their original form and hampering effective characterization analysis. As such, we demonstrate the importance of flash freezing EBC samples immediately after collection and defrosting them shortly before analysis at a temperature that does not affect proteins and peptides (<38°C) in order to effectively preserve NPs in particulate form. This methodology can be applied easily, effectively, and inexpensively to preserve EBC samples for future NP content determination and characterization.
Mots-clé
Welding , Nanoparticles/analysis , Breath Tests/methods
Web of science
Open Access
Oui
Création de la notice
24/03/2016 16:40
Dernière modification de la notice
08/05/2019 17:38
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