The genes for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) are tandemly arranged on chromosome 17 of the mouse

Détails

ID Serval
serval:BIB_40800E9FCDB1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The genes for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) are tandemly arranged on chromosome 17 of the mouse
Périodique
Nucleic Acids Research
Auteur(s)
Nedospasov  S. A., Hirt  B., Shakhov  A. N., Dobrynin  V. N., Kawashima  E., Accolla  R. S., Jongeneel  C. V.
ISSN
0305-1048 (Print)
Statut éditorial
Publié
Date de publication
10/1986
Volume
14
Numéro
19
Pages
7713-25
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 10
Résumé
We have isolated clones containing the gene for tumor necrosis factor (TNF-alpha) from a mouse genomic library. Four out of five clones containing the TNF-alpha gene also hybridized to a human lymphotoxin (TNF-beta) probe. We constructed a restriction enzyme cleavage map of a 6.4 kb region from one of the genomic clones. From partial sequencing data and hybridizations with exon-specific oligonucleotide probes, we conclude that this region contains the mouse TNF-alpha and TNF-beta genes in a tandem arrangement, that they are separated by only about 1100 bases, and that their intron-exon structure is very similar to that seen in man. We probed genomic blots of DNA from human/mouse hybrids containing single mouse chromosomes for the presence of the mouse TNF genes. The results show that the genes are located on mouse chromosome 17, which also contains the major histocompatibility complex. Therefore, both the mouse and the human TNF genes are tandemly arranged and located on the same chromosome as the MHC.
Mots-clé
Animals Base Sequence *Chromosome Mapping DNA/analysis Gene Expression Regulation Glycoproteins/*genetics Humans Lymphotoxin-alpha/*genetics Mice Nucleic Acid Hybridization Sequence Homology, Nucleic Acid Tumor Necrosis Factor-alpha
Pubmed
Web of science
Création de la notice
24/01/2008 16:39
Dernière modification de la notice
03/03/2018 16:28
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