Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Détails

ID Serval
serval:BIB_40678CC312D4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection
Périodique
Optics letters
Auteur(s)
Kühn J., Montfort F., Colomb T., Rappaz B., Moratal C., Pavillon N., Marquet P., Depeursinge C.
ISSN
0146-9592
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
34
Numéro
5
Pages
653-655
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 mum axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.
Pubmed
Web of science
Création de la notice
13/03/2009 10:57
Dernière modification de la notice
20/08/2019 13:38
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