Draft Genome Assembly and Population Genetics of an Agricultural Pollinator, the Solitary Alkali Bee (Halictidae: Nomia melanderi).

Détails

Ressource 1Télécharger: 625.full.pdf (1352.41 [Ko])
Etat: Serval
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_403A684B1A7B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Draft Genome Assembly and Population Genetics of an Agricultural Pollinator, the Solitary Alkali Bee (Halictidae: Nomia melanderi).
Périodique
G3-Genes-Genome-Genetics
Auteur(s)
Kapheim K.M., Pan H., Li C., Blatti C., Harpur B.A., Ioannidis P., Jones B.M., Kent C.F., Ruzzante L., Sloofman L., Stolle E., Waterhouse R.M., Zayed A., Zhang G., Wcislo W.T.
ISSN
2160-1836 (Electronic)
ISSN-L
2160-1836
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Volume
9
Numéro
3
Pages
625-634
Langue
anglais
Résumé
Alkali bees (Nomia melanderi) are solitary relatives of the halictine bees, which have become an important model for the evolution of social behavior, but for which few solitary comparisons exist. These ground-nesting bees defend their developing offspring against pathogens and predators, and thus exhibit some of the key traits that preceded insect sociality. Alkali bees are also efficient native pollinators of alfalfa seed, which is a crop of major economic value in the United States. We sequenced, assembled, and annotated a high-quality draft genome of 299.6 Mbp for this species. Repetitive content makes up more than one-third of this genome, and previously uncharacterized transposable elements are the most abundant type of repetitive DNA. We predicted 10,847 protein coding genes, and identify 479 of these undergoing positive directional selection with the use of population genetic analysis based on low-coverage whole genome sequencing of 19 individuals. We found evidence of recent population bottlenecks, but no significant evidence of population structure. We also identify 45 genes enriched for protein translation and folding, transcriptional regulation, and triglyceride metabolism evolving slower in alkali bees compared to other halictid bees. These resources will be useful for future studies of bee comparative genomics and pollinator health research.
Mots-clé
alternative pollinators, population genetics, sociogenomics, solitary bee, transposable elements
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/01/2019 10:14
Dernière modification de la notice
08/05/2019 17:36
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