Article: article from journal or magazin.
Probing the interactions of NK cell receptors with ligand expressed in trans and cis.
Methods in Molecular Biology
Certain receptors on natural killer (NK) cells, which are specific for MHC class I (MHC-I) molecules, do not only interact with ligand expressed on opposing cell membranes (in trans) but also interact with those on the same cell membrane (in cis). Cis interactions have been demonstrated for only a small number of cell surface receptors. However, this has not been tested systematically, raising the possibility that additional receptors may be able to bind ligand expressed in cis. Here we describe a number of approaches to evaluate trans and cis binding of the Ly49A NK cell receptor to its H-2D(d) ligand. These procedures should facilitate the investigation of cis/trans interactions of other receptor-ligand pairs and simplify the analysis of NK cell receptor variants.
Animals, Cell Adhesion, Cell Line, Cell Membrane/metabolism, Gene Expression Regulation, Killer Cells, Natural/cytology, Killer Cells, Natural/metabolism, Ligands, NK Cell Lectin-Like Receptor Subfamily A/metabolism, Protein Binding, Solubility, Substrate Specificity
Web of science
Last modification date