Calcium imaging of odor-evoked responses in the Drosophila antennal lobe.

Détails

ID Serval
serval:BIB_40127477369D
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Calcium imaging of odor-evoked responses in the Drosophila antennal lobe.
Périodique
Journal of Visualized Experiments
Auteur(s)
Silbering A.F., Bell R., Galizia C.G., Benton R.
ISSN
1940-087X (Electronic)
ISSN-L
1940-087X
Statut éditorial
Publié
Date de publication
2012
Numéro
61
Pages
2976
Langue
anglais
Notes
type modifié
Résumé
The antennal lobe is the primary olfactory center in the insect brain and represents the anatomical and functional equivalent of the vertebrate olfactory bulb. Olfactory information in the external world is transmitted to the antennal lobe by olfactory sensory neurons (OSNs), which segregate to distinct regions of neuropil called glomeruli according to the specific olfactory receptor they express. Here, OSN axons synapse with both local interneurons (LNs), whose processes can innervate many different glomeruli, and projection neurons (PNs), which convey olfactory information to higher olfactory brain regions. Optical imaging of the activity of OSNs, LNs and PNs in the antennal lobe - traditionally using synthetic calcium indicators (e.g. calcium green, FURA-2) or voltage-sensitive dyes (e.g. RH414) - has long been an important technique to understand how olfactory stimuli are represented as spatial and temporal patterns of glomerular activity in many species of insects. Development of genetically-encoded neural activity reporters, such as the fluorescent calcium indicators G-CaMP and Cameleon, the bioluminescent calcium indicator GFP-aequorin, or a reporter of synaptic transmission, synapto-pHluorin has made the olfactory system of the fruitfly, Drosophila melanogaster, particularly accessible to neurophysiological imaging, complementing its comprehensively-described molecular, electrophysiological and neuroanatomical properties. These reporters can be selectively expressed via binary transcriptional control systems (e.g. GAL4/UAS, LexA/LexAop, Q system) in defined populations of neurons within the olfactory circuitry to dissect with high spatial and temporal resolution how odor-evoked neural activity is represented, modulated and transformed. Here we describe the preparation and analysis methods to measure odor-evoked responses in the Drosophila antennal lobe using G-CaMP. The animal preparation is minimally invasive and can be adapted to imaging using wide-field fluorescence, confocal and two-photon microscopes.
Mots-clé
Animals, Arthropod Antennae/physiology, Calcium/analysis, Calcium/chemistry, Drosophila melanogaster/physiology, Fluorescent Dyes/chemistry, Green Fluorescent Proteins/chemistry, Microscopy, Fluorescence/methods, Odors, Smell/physiology
Pubmed
Open Access
Oui
Création de la notice
17/01/2013 15:55
Dernière modification de la notice
20/08/2019 13:37
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