Effects of antibacterial agents and drugs monitored by atomic force microscopy.

Détails

ID Serval
serval:BIB_3F833B927F6D
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Effects of antibacterial agents and drugs monitored by atomic force microscopy.
Périodique
Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
Auteur(s)
Longo G., Kasas S.
ISSN
1939-0041 (Electronic)
ISSN-L
1939-0041
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
6
Numéro
3
Pages
230-244
Langue
anglais
Notes
Publication types: Journal Article ; ReviewPublication Status: ppublish
Résumé
Originally invented for topographic imaging, atomic force microscopy (AFM) has evolved into a multifunctional biological toolkit, enabling to measure structural and functional details of cells and molecules. Its versatility and the large scope of information it can yield make it an invaluable tool in any biologically oriented laboratory, where researchers need to perform characterizations of living samples as well as single molecules in quasi-physiological conditions and with nanoscale resolution. In the last 20 years, AFM has revolutionized the characterization of microbial cells by allowing a better understanding of their cell wall and of the mechanism of action of drugs and by becoming itself a powerful diagnostic tool to study bacteria. Indeed, AFM is much more than a high-resolution microscopy technique. It can reconstruct force maps that can be used to explore the nanomechanical properties of microorganisms and probe at the same time the morphological and mechanical modifications induced by external stimuli. Furthermore it can be used to map chemical species or specific receptors with nanometric resolution directly on the membranes of living organisms. In summary, AFM offers new capabilities and a more in-depth insight in the structure and mechanics of biological specimens with an unrivaled spatial and force resolution. Its application to the study of bacteria is extremely significant since it has already delivered important information on the metabolism of these small microorganisms and, through new and exciting technical developments, will shed more light on the real-time interaction of antimicrobial agents and bacteria.
Mots-clé
Anti-Bacterial Agents/pharmacokinetics, Anti-Bacterial Agents/pharmacology, Bacteria/cytology, Bacteria/drug effects, Bacterial Physiological Phenomena/drug effects, Cell Size/drug effects, Micromanipulation/methods, Microscopy, Atomic Force/methods, Molecular Imaging/methods
Pubmed
Web of science
Création de la notice
08/09/2014 13:40
Dernière modification de la notice
03/03/2018 16:25
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