High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization.

Détails

ID Serval
serval:BIB_3F57EF76B170
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Holden S.J., Pengo T., Meibom K.L., Fernandez Fernandez C., Collier J., Manley S.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
111
Numéro
12
Pages
4566-4571
Langue
anglais
Résumé
We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of "Z-ring" organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.
Mots-clé
Caulobacter crescentus/cytology, Caulobacter crescentus/drug effects, Cell Cycle, DNA Damage, DNA, Bacterial/drug effects, DNA, Bacterial/genetics, Microscopy/methods, Mitomycin/pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/02/2014 17:23
Dernière modification de la notice
08/05/2019 17:33
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