An overview of the metabolic differences between Bradyrhizobium japonicum 110 bacteria and differentiated bacteroids from soybean (Glycine max) root nodules: An in vitro 13C-and 31P-nuclear magnetic resonance spectroscopy study

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_3F1A9A9F73B1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An overview of the metabolic differences between Bradyrhizobium japonicum 110 bacteria and differentiated bacteroids from soybean (Glycine max) root nodules: An in vitro 13C-and 31P-nuclear magnetic resonance spectroscopy study
Périodique
FEMS Microbiology Letters
Auteur(s)
Vauclare P., Bligny R., Gout E., Widmer F.
ISSN
0378-1097
ISSN-L
0378-1097
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
343
Numéro
1
Pages
49-56
Langue
anglais
Résumé
Bradyrhizobium japonicum is a symbiotic nitrogen-fixing soil bacteria that induce root nodules formation in legume soybean (Glycine max.). Using 13C- and 31P-nuclear magnetic resonance (NMR) spectroscopy, we have analysed the metabolite profiles of cultivated B.japonicum cells and bacteroids isolated from soybean nodules. Our results revealed some quantitative and qualitative differences between the metabolite profiles of bacteroids and their vegetative state. This includes in bacteroids a huge accumulation of soluble carbohydrates such as trehalose, glutamate, myo-inositol and homospermidine as well as Pi, nucleotide pools and intermediates of the primary carbon metabolism. Using this novel approach, these data show that most of the compounds detected in bacteroids reflect the metabolic adaptation of rhizobia to the surrounding microenvironment with its host plant cells.
Mots-clé
metabolism, NMR, Bradyrhizobium japonicum, rhizobium-legume symbiosis, nitrogen-fixation, plant-cells, accumulation, glutamate, meliloti, betaine, oxygen, dehydrogenase, polyamines, Microbiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/09/2015 10:13
Dernière modification de la notice
20/08/2019 13:36
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