Article: article from journal or magazin.
Modulation of phosphorylation of neuronal cytoskeletal proteins by neuronal depolarization.
Cellular and Molecular Neurobiology
1. The neuronal cytoskeletal protein tau and the carboxy tails of cytoskeletal proteins neurofilament-M (NF-M) and neurofilament-H (NF-H) are phosphorylated on serine residues by the cyclin-dependent kinase cdk-5. 2. In aggregating neuronal-glial cultures we show that veratridine-mediated cation influx causes dephosphorylation of tau, NF-M and NF-H. Dephosphorylation was blocked specifically by cyclosporine A but not by okadiac acid at concentrations up to 200 nM. 3. These results suggest that veratridine-triggered cation influx causes activation of PP-2B (calcineurin) leading to dephosphorylation of these cytoskeletal proteins.
Animals, Blotting, Western, Cells, Cultured, Cytoskeletal Proteins/drug effects, Cytoskeletal Proteins/metabolism, Embryo, Mammalian, Nerve Tissue Proteins/drug effects, Nerve Tissue Proteins/metabolism, Neurofilament Proteins/drug effects, Neurofilament Proteins/metabolism, Neuromuscular Depolarizing Agents/pharmacology, Phosphoric Monoester Hydrolases/drug effects, Phosphoric Monoester Hydrolases/physiology, Phosphorylation/drug effects, Rats, Sodium/metabolism, Veratridine/pharmacology, tau Proteins/metabolism
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