Acid proteinase secreted by Candida tropicalis: virulence in mice of a proteinase negative mutant

Details

Serval ID
serval:BIB_3EB070F4CA7E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Acid proteinase secreted by Candida tropicalis: virulence in mice of a proteinase negative mutant
Journal
Journal of Medical and Veterinary Mycology
Author(s)
Togni  G., Sanglard  D., Monod  M.
ISSN
0268-1218 (Print)
Publication state
Published
Issued date
1994
Volume
32
Number
4
Pages
257-65
Notes
Comparative Study
Journal Article
Abstract
The relationship between the ability to secrete a specific acid proteinase (ACP) by Candida tropicalis in the presence of bovine serum albumin as a nitrogen source and virulence for mice was studied using two stable proteinase-positive and proteinase-negative strains (DSY68 and DSY65), which were constructed from the wild-type pathogenic yeast C. tropicalis ATCC 750. The inactivation of the gene encoding the secreted acid proteinase was produced by targeted gene disruption. Mortality rate was slightly lower in groups of mice infected with the proteinase-negative mutant. All other parameters analysed were similar for two strains of yeast. Our results therefore conclude that the ACP secreted by C. tropicalis did not contribute significantly to fungal virulence in systemic infections.
Keywords
Animals Aspartic Endopeptidases/*biosynthesis/genetics Candida/*enzymology/genetics/*pathogenicity Candidiasis/microbiology/pathology/*physiopathology Mice Mutagenesis Species Specificity Ultraviolet Rays Virulence
Pubmed
Web of science
Create date
25/01/2008 16:46
Last modification date
20/08/2019 13:35
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