The retinoblastoma-like protein p130 is involved in the determination of reserve cells in differentiating myoblasts.

Details

Serval ID
serval:BIB_3DC318EA9B62
Type
Article: article from journal or magazin.
Collection
Publications
Title
The retinoblastoma-like protein p130 is involved in the determination of reserve cells in differentiating myoblasts.
Journal
Current Biology
Author(s)
Carnac G., Fajas L., L'honoré A., Sardet C., Lamb N.J., Fernandez A.
ISSN
0960-9822 (Print)
ISSN-L
0960-9822
Publication state
Published
Issued date
2000
Volume
10
Number
9
Pages
543-546
Language
english
Abstract
During skeletal muscle differentiation, a subset of myoblasts remains quiescent and undifferentiated but retains the capacity to self-renew and give rise to differentiating myoblasts [1] [2] [3]: this sub-population of muscle cells was recently termed 'reserve cells' [3]. In order to characterise genes that can regulate the ratio between reserve cells and differentiating myoblasts, we examined members of the retinoblastoma tumor suppressor family - Rb, p107 and p130 - an important family of negative regulators of E2F transcription factors and cell cycle progression [4]. Although pRb and p107 positively regulate muscle cell differentiation [5] [6] [7], the role of p130 in muscle cells remains unknown. We show here that p130 (protein and mRNA), but neither pRb nor p107, preferentially accumulates during muscle differentiation in reserve cells. Also, p130 is the major Rb-family protein present in E2F complexes in this sub-population of cells. Although forced expression of either p130 or pRb in mouse C2 myoblasts efficiently blocked cell cycle progression, only p130 inhibited the differentiation program. Furthermore, muscle cells overexpressing p130 had reduced levels of the muscle-promoting factor MyoD. In addition, p130 repressed the transactivation capacity of MyoD, an effect abolished by co-transfection of pRb. Thus, we propose that p130, by blocking cell cycle progression and differentiation, could be part of a specific pathway that defines a pool of reserve cells during terminal differentiation.
Keywords
Animals, Carrier Proteins, Cell Cycle, Cell Cycle Proteins, Cell Differentiation, Cell Line, DNA-Binding Proteins, E2F Transcription Factors, Gene Expression Regulation, Mice, Muscle, Skeletal/cytology, MyoD Protein/genetics, Phosphoproteins/genetics, Phosphoproteins/metabolism, Proteins, Retinoblastoma-Binding Protein 1, Retinoblastoma-Like Protein p130, Stem Cells/cytology, Transcription Factor DP1, Transcription Factors/metabolism, Transcriptional Activation
Pubmed
Web of science
Open Access
Yes
Create date
07/03/2013 16:08
Last modification date
20/08/2019 13:34
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