Monitoring of hemoglobin and erythropoiesis-related mRNA with dried blood spots in athletes and patients.

Details

Serval ID
serval:BIB_3D12A0F09E1E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Monitoring of hemoglobin and erythropoiesis-related mRNA with dried blood spots in athletes and patients.
Journal
Bioanalysis
Author(s)
Loria F., Stutz A.P., Rocca A., Grabherr S., Kuuranne T., Pruijm M., Leuenberger N.
ISSN
1757-6199 (Electronic)
ISSN-L
1757-6180
Publication state
Published
Issued date
03/2022
Peer-reviewed
Oui
Volume
14
Number
5
Pages
241-251
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Aim: We assessed the feasibility of using hematological parameters (such as hemoglobin and reticulocyte mRNA) in dried blood spot (DBS) samples to test athletes for doping and to improve patient care. Methods: Hemoglobin and erythropoiesis-related mRNAs were measured in venous blood and DBSs from both healthy athletes and hemodialysis patients. Results: We accurately measured hemoglobin changes over time in both venous blood and DBS samples. Combining hemoglobin and mRNA analyses, we detected erythropoietin injection in DBSs more sensitively and with higher efficiency by using the DBS OFF-score than by using the athlete biological passport OFF-score. Conclusion: DBS-based measurements are practical for calculating hemoglobin levels and athlete biological passport OFF-scores. This approach may help detect blood doping and help predict patient response to EPO.
Keywords
anti-doping, dried blood spots, hemodialysis, hemoglobin, mRNA, reticulocyte
Pubmed
Web of science
Create date
18/02/2022 14:37
Last modification date
30/07/2022 5:36
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