Hydrophilic interaction liquid chromatography and accurate mass measurement for quantification and confirmation of morphine, codeine and their glucuronide conjugates in human urine.

Details

Serval ID
serval:BIB_3D100D42C946
Type
Article: article from journal or magazin.
Collection
Publications
Title
Hydrophilic interaction liquid chromatography and accurate mass measurement for quantification and confirmation of morphine, codeine and their glucuronide conjugates in human urine.
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Author(s)
Kolmonen M., Leinonen A., Kuuranne T., Pelander A., Ojanperä I.
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Publication state
Published
Issued date
01/11/2010
Volume
878
Number
29
Pages
2959-2966
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article
Publication Status: ppublish
Abstract
A hydrophilic interaction liquid chromatography-time-of-flight mass spectrometry (HILIC-TOFMS) method for the quantification and confirmation of morphine (M), codeine (C), morphine-3-glucuronide (M3G), morphine-6-glucuronide (M6G) and codeine-6-glucuronide (C6G) is presented. The method was validated in terms of specificity, selectivity, extraction recovery, accuracy, repeatability, linearity and matrix effect. After a straightforward sample preparation by solid phase extraction (SPE) the compounds were analyzed directly without the need for hydrolysis, solvent transfer, evaporation or reconstitution. The HILIC technique provided good chromatographic separation which was critical for isomers M3G and M6G. The analytes were detected after electrospray ionization (ESI) in positive mode with mass accuracies below 2 mDa using a 5-mDa window. A measurement range of 50-5000 ng/ml was applied for calibration using deuterated analogs as internal standards. The precision of the method was 5.7% and 10.2% (RSD) within and between days, respectively. The applicability of the method was demonstrated with authentic urine samples known to contain codeine and/or morphine and their intact glucuronide conjugates. Identification of the analytes was based on in-source collision induced dissociation (ISCID), applying three diagnostic ions with accurate mass.

Keywords
Chromatography, Liquid/methods, Codeine/chemistry, Codeine/urine, Glucuronides/chemistry, Glucuronides/urine, Humans, Hydrophobic and Hydrophilic Interactions, Isomerism, Molecular Weight, Morphine/chemistry, Morphine/urine, Spectrometry, Mass, Electrospray Ionization/methods
Pubmed
Web of science
Create date
02/05/2017 14:28
Last modification date
20/08/2019 13:33
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