Revisiting a pollen-transmitted ilarvirus previously associated with angular mosaic of grapevine

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_3D06895D886A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Revisiting a pollen-transmitted ilarvirus previously associated with angular mosaic of grapevine
Journal
Virus Research
Author(s)
Mahillon Mathieu, Brodard Justine, Schoen Ruben, Botermans Marleen, Dubuis Nathalie, Groux Raphaël, Pannell John R., Blouin Arnaud G., Schumpp Olivier
ISSN
0168-1702
ISSN-L
0168-1702
Publication state
Published
Issued date
06/2024
Peer-reviewed
Oui
Volume
344
Pages
199362
Language
english
Abstract
We report the characterization of a novel tri-segmented RNA virus infecting Mercurialis annua, a common crop weed and model species in plant science. The virus, named "Mercurialis latent virus" (MeLaV) was first identified in a mixed infection with the recently described Mercurialis orthotospovirus 1 (MerV1) on symptomatic plants grown in glasshouses in Lausanne (Switzerland). Both viruses were found to be transmitted by Thrips tabaci, which presumably help the inoculation of infected pollen in the case of MeLaV. Complete genome sequencing of the latter revealed a typical ilarviral architecture and close phylogenetic relationship with members of the Ilarvirus subgroup 1. Surprisingly, a short portion of MeLaV replicase was found to be identical to the partial sequence of grapevine angular mosaic virus (GAMV) reported in Greece in the early 1990s. However, we have compiled data that challenge the involvement of GAMV in angular mosaic of grapevine, and we propose alternative causal agents for this disorder. In parallel, three highly-conserved MeLaV isolates were identified in symptomatic leaf samples in The Netherlands, including a herbarium sample collected in 1991. The virus was also traced in diverse RNA sequencing datasets from 2013-2020, corresponding to transcriptomic analyses of M. annua and other plant species from five European countries, as well as metaviromics analyses of bees in Belgium. Additional hosts are thus expected for MeLaV, yet we argue that infected pollen grains have likely contaminated several sequencing datasets and may have caused the initial characterization of MeLaV as GAMV.
Keywords
Cancer Research, Infectious Diseases, Virology
Pubmed
Open Access
Yes
Create date
22/03/2024 14:37
Last modification date
23/04/2024 7:10
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