Article: article from journal or magazin.
IL-2-mediated T cell proliferation in humans is blocked by a monoclonal antibody directed against monomorphic determinants of HLA-DR antigens
Journal of Experimental Medicine
Journal Article --- Old month value: Feb 1
We have studied the effect on the interleukin (IL-) 2-dependent human T cell growth of two distinct monoclonal antibodies (Mab), D1-12 and 4F2, with specificity for common determinant of human Ia antigens and for a differentiation antigen expressed on all activated T cells, respectively. Strong inhibition of cell growth was found in cultures supplemented with the anti-Ia D1-12 Mab but not in cultures supplemented with 4F2 Mab. These results were obtained when either total mixed leukocyte culture (MLC) T cells or an MLC-derived T cell clone were used as indicator cell systems for IL-2 activity. The inhibition of cell growth appears to be mediated by a direct interaction of D1-12 Mab with the cells and not by a direct inactivation of the growth factor, as addition of the antibody to murine MLC T cells, which do not express the determinant defined by D1-12 Mab, resulted in no inhibition of their proliferation induced by the same source of human IL-2.
Animals Antibodies, Monoclonal/*immunology Antibody-Dependent Cell Cytotoxicity Binding, Competitive Epitopes Histocompatibility Antigens Class II/immunology Humans Interleukin-2/*pharmacology Lymphocyte Activation/*drug effects Lymphocyte Culture Test, Mixed Lymphokines/*pharmacology Mice Mice, Inbred C57BL Mice, Inbred DBA T-Lymphocytes/*immunology
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