The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1.

Détails

ID Serval
serval:BIB_3C4C179BA946
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1.
Périodique
Molecular and Cellular Biology
Auteur(s)
Wong M.W., Henry R.W., Ma B., Kobayashi R., Klages N., Matthias P., Strubin M., Hernandez N.
ISSN
0270-7306[print], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
01/1998
Volume
18
Numéro
1
Pages
368-377
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The human RNA polymerase II and III snRNA promoters have similar enhancers, the distal sequence elements (DSEs), and similar basal promoter elements, the proximal sequence elements (PSEs). The DSE, which contains an octamer motif, binds broadly expressed activator Oct-1. The PSE binds a multiprotein complex referred to as SNAPc or PTF. On DNAs containing both an octamer site and a PSE, Oct-1 and SNAPc bind cooperatively. SNAPc consists of at least four stably associated subunits, SNAP43, SNAP45, SNAP50, and SNAP190. None of the three small subunits, which have all been cloned, can bind to the PSE on their own. Here we report the isolation of cDNAs corresponding to the largest subunit of SNAPc, SNAP190. SNAP190 contains an unusual Myb DNA binding domain consisting of four complete repeats (Ra to Rd) and a half repeat (Rh). A truncated protein consisting of the last two SNAP190 Myb repeats, Rc and Rd, can bind to the PSE, suggesting that the SNAP190 Myb domain contributes to recognition of the PSE by the SNAP complex. SNAP190 is required for snRNA gene transcription by both RNA polymerases II and III and interacts with SNAP45. In addition, SNAP190 interacts with Oct-1. Together, these results suggest that the largest subunit of the SNAP complex is involved in direct recognition of the PSE and is a target for the Oct-1 activator. They also provide an example of a basal transcription factor containing a Myb DNA binding domain.
Mots-clé
Amino Acid Sequence, DNA-Binding Proteins/genetics, Hela Cells, Host Cell Factor C1, Humans, Molecular Sequence Data, Octamer Transcription Factor-1, Oncogenes, RNA Polymerase I/genetics, RNA Polymerase II/genetics, RNA, Small Nuclear/genetics, Sequence Alignment, Transcription Factors/genetics, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 14:32
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