Early-stage lung cancer is increasingly being managed by sublobar resection, which offers equivalent oncological results to lobectomy for lesions of less than 2 cm. However, isolated resection of the laterobasal segment (S9) is a difficult procedure and has rarely been reported via a uniportal video-assisted thoracoscopic (VATS) approach. The two key challenges are, first, the segmental arterial supply is deeply located in the lung parenchyma, making the dissection difficult, and second, the cuboidal shape of the lateral basilar segment hampers the identification of the intersegmental plane.