Biliary obstruction dissipates bioelectric sinusoidal-canalicular barrier without altering taurocholate uptake
Details
Serval ID
serval:BIB_3B5EA6369640
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Biliary obstruction dissipates bioelectric sinusoidal-canalicular barrier without altering taurocholate uptake
Journal
American Journal of Physiology
ISSN
0193-1857
0002-9513 (Print)
0002-9513 (Print)
Publication state
Published
Issued date
02/1989
Volume
256
Number
2 Pt 1
Pages
G312-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
To study immediate events during extrahepatic cholestasis, we investigated the effect of short-term biliary obstruction on the bioelectrical sinusoidal-canalicular barrier in the rat using molecular weight-matched uncharged and negatively charged inert solute pairs. The bioelectrical barrier averaged -22 +/- 5 and -18 +/- 4 mV (NS) using the pair carboxy-/methoxyinulin and ferrocyanide/sucrose, respectively. After a 20-min biliary obstruction both decreased by 61 and 11%, respectively, but only the large molecular weight pair (the inulins) returned to base line after release of the obstruction. Inert solute clearances were increased after short biliary obstruction depending on molecular size and negative charge (ferrocyanide greater than sucrose greater than carboxyinulin greater than inulin), suggesting that both permeability and bioelectrical barriers were affected by obstruction. The hepatic extraction in vivo of a passively transported drug not excreted into bile (D-propranolol) was not affected by obstruction, whereas that of an actively transported drug (glycocholate) decreased from 66 +/- 8 to 41 +/- 20% during biliary obstruction (P less than 0.01). Unidirectional transfer of glycocholate was not affected by short-term biliary obstruction in the situ perfused rat liver; however, 2 min after [14C]glycocholate administration, increased return was observed in hepatic venous effluent in obstructed animals. Our findings demonstrate a loss of the bioelectrical barrier immediately after short-term biliary obstruction. Decreased hepatic extraction in the view of unaltered sinusoidal uptake demonstrates regurgitation of bile into blood during short-term biliary obstruction.
Keywords
Animals
Bile/*secretion
Carbon Radioisotopes/diagnostic use
Cholestasis, Extrahepatic/*physiopathology
Chromium Radioisotopes/diagnostic use
Disease Models, Animal
Ferrocyanides/diagnostic use
Glycocholic Acid/metabolism
Inulin/diagnostic use
Male
Rats
Rats, Inbred Strains
Reference Values
Sucrose/diagnostic use
Taurocholic Acid/*metabolism
Technetium/diagnostic use
Tritium/diagnostic use
Pubmed
Web of science
Create date
24/01/2008 17:41
Last modification date
20/08/2019 14:31