The determination of ethyl glucuronide in hair: Experiences from nine consecutive interlaboratory comparison rounds.

Détails

ID Serval
serval:BIB_3B5484AAB5F5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The determination of ethyl glucuronide in hair: Experiences from nine consecutive interlaboratory comparison rounds.
Périodique
Forensic science international
Auteur(s)
Becker R.,  I., Sporkert F., Baumgartner M.
ISSN
1872-6283 (Electronic)
ISSN-L
0379-0738
Statut éditorial
Publié
Date de publication
07/2018
Peer-reviewed
Oui
Volume
288
Pages
67-71
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The increasing request for hair ethyl glucuronide (HEtG) in alcohol consumption monitoring according to cut-off levels set by the Society of Hair Testing (SoHT) has triggered a proficiency testing program based on interlaboratory comparisons (ILC). Here, the outcome of nine consecutive ILC rounds organised by the SoHT on the determination of HEtG between 2011 and 2017 is summarised regarding interlaboratory reproducibility and the influence of procedural variants. Test samples prepared from cut hair (1mm) with authentic (in-vivo incorporated) and soaked (in-vitro incorporated) HEtG concentrations up to 80pg/mg were provided for 27-35 participating laboratories. Laboratory results were evaluated according to ISO 5725-5 and provided robust averages and relative reproducibility standard deviations typically between 20 and 35% in reasonable accordance with the prediction of the Horwitz model. Evaluation of results regarding the analytical techniques revealed no significant differences between gas and liquid chromatographic methods In contrast, a detailed evaluation of different sample preparations revealed significantly higher average values in case when pulverised hair is tested compared to cut hair. This observation was reinforced over the different ILC rounds and can be attributed to the increased acceptance and routine of hair pulverisation among laboratories. Further, the reproducibility standard deviations among laboratories performing pulverisation were on average in very good agreement with the prediction of the Horwitz model. Use of sonication showed no effect on the HEtG extraction yield.
Mots-clé
Alcohol Drinking, Biomarkers/analysis, Chromatography, Gas, Chromatography, Liquid, Forensic Toxicology/methods, Glucuronates/analysis, Hair/chemistry, Humans, Laboratories, Mass Spectrometry, Reproducibility of Results, Specimen Handling/methods, Substance Abuse Detection/methods, Ethyl glucuronide, Interlaboratory comparison, Proficiency testing, Pulverization, Reproducibility, Society of hair testing
Pubmed
Web of science
Création de la notice
12/05/2018 10:25
Dernière modification de la notice
20/08/2019 14:31
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