Increased efficiency of GroE-assisted protein folding by manganese ions.

Détails

ID Serval
serval:BIB_3A8BA211005A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Increased efficiency of GroE-assisted protein folding by manganese ions.
Périodique
Journal of Biological Chemistry
Auteur(s)
Diamant S., Azem A., Weiss C., Goloubinoff P.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1995
Volume
270
Numéro
47
Pages
28387-28391
Langue
anglais
Résumé
This study addresses the role of ATP-bound and free Mg2+ and Mn2+ ions in the activation and modulation of chaperonin-assisted refolding of urea-denatured malate dehydrogenase. As compared with Mg2+, Mn2+ ions caused a significant increase in the rate of GroE-assisted malate dehydrogenase refolding and, concomitantly, a decrease in the rate of ATP hydrolysis. Moreover, Mn2+ increases the affinity of GroES for GroEL, even in the presence of saturating amounts of Mg2+. Chemical cross-linking showed that lower concentrations of Mn-ATP as compared with Mg-ATP are needed to form both asymmetric GroEL14GroES7 and symmetric GroEL14(GroES7)2 particles. The manganese-dependent increase in the rate of protein folding concurred with a specific increase in the amount of symmetric GroEL14-(GroES7)2 particles detected in a chaperonin solution. Thus, Mn2+ is a cofactor that can markedly increase the efficiency of the chaperonin reaction in vitro. Mn2+ ions can serve as an important tool for analyzing the molecular mechanism and the structure of chaperonins.
Mots-clé
Adenosine Triphosphate/metabolism, Adenosine Triphosphate/pharmacology, Animals, Bacterial Proteins/metabolism, Chaperonin 10/metabolism, Chaperonin 60/metabolism, Chaperonins, Cross-Linking Reagents, Escherichia coli Proteins, Heat-Shock Proteins/metabolism, Kinetics, Magnesium/pharmacology, Malate Dehydrogenase/chemistry, Malate Dehydrogenase/drug effects, Manganese/pharmacology, Mitochondria, Heart/enzymology, Protein Folding, Swine
Pubmed
Web of science
Création de la notice
24/01/2008 21:02
Dernière modification de la notice
20/08/2019 14:30
Données d'usage