Heterogeneity assessment of functional T cell avidity.

Détails

Ressource 1Télécharger: srep44320.pdf (1176.89 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_39C885ED43A7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Heterogeneity assessment of functional T cell avidity.
Périodique
Scientific reports
Auteur(s)
Ioannidou K., Baumgaertner P., Gannon P.O., Speiser M.F., Allard M., Hebeisen M., Rufer N., Speiser D.E.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
13/03/2017
Peer-reviewed
Oui
Volume
7
Pages
44320
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
The potency of cellular immune responses strongly depends on T cell avidity to antigen. Yet, functional avidity measurements are rarely performed in patients, mainly due to the technical challenges of characterizing heterogeneous T cells. The mean functional T cell avidity can be determined by the IFN-γ Elispot assay, with titrated amounts of peptide. Using this assay, we developed a method revealing the heterogeneity of functional avidity, represented by the steepness/hillslope of the peptide titration curve, documented by proof of principle experiments and mathematical modeling. Our data show that not only natural polyclonal CD8 T cell populations from cancer patients, but also monoclonal T cells differ strongly in their heterogeneity of functional avidity. Interestingly, clones and polyclonal cells displayed comparable ranges of heterogeneity. We conclude that besides the mean functional avidity, it is feasible and useful to determine its heterogeneity (hillslope) for characterizing T cell responses in basic research and patient investigation.
Mots-clé
CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/metabolism, Cell Survival/immunology, Clone Cells/immunology, Clone Cells/metabolism, Enzyme-Linked Immunospot Assay/methods, Epitopes, T-Lymphocyte/immunology, Feasibility Studies, Humans, Immunity, Cellular/immunology, Interferon-gamma/immunology, Interferon-gamma/metabolism, Lymphocyte Activation/immunology, Reproducibility of Results
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/03/2017 19:18
Dernière modification de la notice
20/08/2019 14:29
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