The majority of autologous cytolytic T-lymphocyte clones derived from peripheral blood lymphocytes of a melanoma patient recognize an antigenic peptide derived from gene Pmel17/gp100

Details

Serval ID
serval:BIB_39A04959572C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The majority of autologous cytolytic T-lymphocyte clones derived from peripheral blood lymphocytes of a melanoma patient recognize an antigenic peptide derived from gene Pmel17/gp100
Journal
Journal of Investigative Dermatology
Author(s)
Zarour  H., De Smet  C., Lehmann  F., Marchand  M., Lethe  B., Romero  P., Boon  T., Renauld  J. C.
ISSN
0022-202X (Print)
Publication state
Published
Issued date
07/1996
Volume
107
Number
1
Pages
63-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
Anti-melanoma cytolytic T-lymphocyte (CTL) clones were derived from peripheral blood lymphocytes of HLA-A2 melanoma patient LB265 after stimulation with the autologous tumor cell line LB265-MEL, which showed high expression of melanocyte-lineage specific genes. Of 55 CTL clones, 46 recognized HLA-A2-restricted antigens. These 46 CTL clones were studied for their ability to specifically release tumor necrosis factor in the presence of COS cells cotransfected with the HLA-A2 gene and the cDNA of either tyrosinase, Melan-A/MART1, Pmel17/gpl00, gp75/TRP1, or MSH receptor. Six CTL clones recognized the Melan-A/MART1 antigen, whereas the remaining 40 CTL clones recognized a Pmel17/gp100 antigen. These 40 anti-PmelI7/gpl00 CTL clones were all able to lyse T2 cells pulsed with the antigenic peptide YLEPGPVTA, as previously reported. The T-cell receptor beta chain hypervariable region was sequenced and found to be identical in the 15 CTL clones analyzed. Taken together, these data show a high frequency of Pmell7/gp100-specific T cells in autologous antitumor CTL clones derived from peripheral blood of a melanoma patient.
Keywords
Amino Acid Sequence Antigens, Neoplasm/*immunology Base Sequence Clone Cells Female *Genes HLA-A2 Antigen/*genetics Humans Melanoma/blood/*immunology Molecular Sequence Data Oligonucleotide Probes/genetics Receptors, Antigen, T-Cell, alpha-beta/genetics T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:28
Last modification date
20/08/2019 13:29
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