Article: article from journal or magazin.
Determination of chlorzoxazone and 6-hydroxychlorzoxazone in plasma by gas chromatography--mass spectrometry.
Journal of Chromatography. B, Biomedical Sciences and Applications
Publication types: Journal ArticlePublication Status: ppublish
A gas chromatographic-mass spectrometric method is presented which allows the determination of chlorzoxazone and 6-hydroxychlorzoxazone after derivatization with the reagent N-tert.-butyldimethylsilyl-N-methyltrifluoroacetamide. No interference was observed from endogenous compounds following the extraction of plasma samples from six different human subjects. The standard curves were linear over a working range of 20 to 4000 ng/ml and of 20 to 1000 ng/ml for chlorzoxazone and 6-hydroxychlorzoxazone, respectively. Recoveries ranged from 65 to 97% for the two compounds and intra- and inter-day coefficients of variation were always less than 9%. The limit of quantitation of the method was found to be 5 ng/ml for the two compounds, hence allowing its use for single low dose pharmacokinetics.
Acetamides, Chlorzoxazone/analogs & derivatives, Chlorzoxazone/blood, Fluoroacetates, Gas Chromatography-Mass Spectrometry, Humans, Muscle Relaxants, Central/blood, Organosilicon Compounds, Sensitivity and Specificity
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